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Luna的测定比现有方法快四倍测试水质

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Christopher Tison, PhD, Senior Research Scientist and Biomedical Technologies Team Lead
纳撒尼尔·塔利，副科学家
Biomedical Technologies Group

Detection of大肠杆菌效率低下且昂贵

The presence of大肠杆菌（大肠杆菌）是水质更关键的指标之一，用作粪便污染的直接指标。然而，现有的检测方法昂贵，耗时，需要实验室专业知识，并花费最多48小时才能完成。另外，许多现有的技术要检测大肠杆菌are unable to distinguish between viable (live) and dead or stressed bacteria, a critical test requirement following disinfection in common water treatment processes such as UV irradiation. Luna is therefore developing a small, portable, rapid and easy to use assay system known as “E.coREADi™” to detect viable fecal coliforms in treated wastewater discharge or other water samples. Luna’s solution is based on a streamlined luminescent detection method in a self-contained syringe and filter device.

Luna.’s fecal coliform detection assay kit known as E.coREADi™ is designed for rapid E. coli detection and quantification.
Luna.’s fecal coliform detection assay kit known as E.coREADi™ is designed for rapid E. coli detection and quantification.

Luna正在启用检测大肠杆菌with Unique Assay Design

Luna的粪便大肠杆菌检测平台基于独特的基于滤光器的反应室，允许以包含和简单的方式进行测定的所有步骤。使用模拟和实际废水建立了基于滤波器的方法的可行性，并开发了“一体化”格式以允许未来的自动化。LUNA的平台可以跨越10毫升至10L的样品，特定于大肠杆菌in the presence of non-fecal coliforms, and has been demonstrated to be effective in detection of chlorine and nutrient stress organisms.

Luna.has currently demonstrated that a 5 hour total assay time is sufficient for detection of approximately 3 individual大肠杆菌100ml样品中的细菌。这比最先进的现有测定速度快约为13至19小时，并且不到15分钟的动手时间，这与现有标准相当。The E.coREADi™ assay is currently in transition from a laboratory assay to an easy-to-use, low-cost, and quantitative disposable assay kit.

E. Coli检测的信号强度与测定时间用于使用EPA认可的标准方法调查的不同细菌起始载荷。Luna的EcoReadi™测定可以在少于5小时的试验时间内检测到100ml水中的3个单独的活细菌细胞。
E. Coli检测的信号强度与测定时间用于使用EPA认可的标准方法调查的不同细菌起始载荷。Luna的EcoReadi™测定可以在少于5小时的试验时间内检测到100ml水中的3个单独的活细菌细胞。

废水分析的未来

Luna的生化氧需求生物传感器测量与稳定的生物包封微生物的有机含量和代谢活性成比例的氧耗尽。
Luna的生化氧需求生物传感器测量与稳定的生物包封微生物的有机含量和代谢活性成比例的氧耗尽。

In addition to the detection of大肠杆菌, Luna is also developing state-of-the-art sensors for the detection of other critical wastewater quality metrics such as biological oxygen demand, or BOD. The BOD is an indicator of the total organic concentration in a water sample as represented by the oxygen required for biological organisms to break that organic matter down.

Luna旨在通过开发一次性，自动化和微流体盒的生物传感器来彻底改变BOD分析，以在实验室或现场环境中提供水样的总机质近实时分析。Luna的技术将封装在生物相容性基质中的微生物赋予赋予的活性长期和室温保存，以及氧敏感的地球嵌入式荧光薄膜。由于微生物代谢与可用营养源成比例，所以样品水中的氧耗水可以直接与有机含量相关。目前的标准测定，BOD5., takes five days to complete and requires substantial laboratory space, equipment, and highly trained personnel for testing.迄今为止，LUNA在环境温度下储存长达6个月的环境温度后，LUNA开发了具有微生物稳定的Benchtop生物传感器，并且可以在不到30分钟的时间内精确地监测来自当地水处理设施的流出物体！

Luna.’s BOD sensor utilizes a fluorescent oxygen-sensitive polymer thin film and encapsulated microorganisms. Here, we demonstrate the linear sensitivity of the sensing film in water samples of relevant dissolved oxygen concentrations (left), as well as an organic content-dependent response of the microbes as determined by oxygen consumption and detected with the thin film (right).
Luna.’s BOD sensor utilizes a fluorescent oxygen-sensitive polymer thin film and encapsulated microorganisms. Here, we demonstrate the linear sensitivity of the sensing film in water samples of relevant dissolved oxygen concentrations (left), as well as an organic content-dependent response of the microbes as determined by oxygen consumption and detected with the thin film (right).

监测水质对市政至关重要water treatment facilities, environmental testing agencies, and related industries in the civilian and military markets. Driven by increasing environmental regulation and industrial demands, the global water industry is a hundreds of billions dollar market. Similarly, “monitoring, control, and analytics” of wastewater treatment comprised approximately 3.0 of the 220 billion dollars in annual capital and operating expenditures for the municipal wastewater industry in 2014.

Join us this May at the Gaylord National Resort & Convention Center in National Harbor, MD where we will be presenting our water analysis development efforts at theSBIR过渡的海军论坛（FST）。我们的演讲将于2016年5月16日星期一11:50 est的马里兰州3会议室。您也可以在展位＃54找到我们。听到我们的传感器并与我们讨论这款令人兴奋的技术！